P-312 Automated sperm selection software (SiD) is as good as human selection for ICSI

Abstract Study question Automated sperm selection or human eye-based selection, does it make a difference on IVF lab outcomes? Summary answer Single by the AI SiD was as proficient highly experienced embryologists and embryo development may have improved for inexperienced embryologists. What is known already The implementation of artificial intelligence in reproductive medicine has proven effective variety applications. One application this technology single during ICSI. computer assisted program SiD, designed to grade sperm, real-time, based motility parameters transforming qualitative assessments into quantitative score. SiD’s assistance can be used select most optimal expected bypass user bias, fatigue, limited training, while also optimising time. However, there are studies correlating usage with ICSI outcomes. design, size, duration Between August November 2022, sibling MII oocytes were randomly divided two groups; 1-ICSIgroup: performed selected embryologist 2- SID-ICSIgroup: using software. Priority given classified “Best” SID software “good” considered second choice. Morphology before both groups. Embryos cultured 6 days same conditions. Participants/materials, setting, methods Sperm preparation microfluidic chamber (Zymot™). previously described, following routine procedures. Biological outcomes analysed groups compared including fertilization rate, cleavage blastocyst top quality rate rate. seniority. Finally, morphokinetics through manual TLT annotations. Main results role chance A total 226 included “ICSI group” 227 “SID-ICSI group. non significant trend towards better observed SID-ICSI group all biological (81,5% vs 80,5%), (98,4% 97,3%), day 3 (77,8% 73,1%), at (50,8% 50,0%), 5 (49,7% 45,1%), good (48,1% 46,2%), (25,4% 23,1%). Similarly, non-significant increase these when comparing junior embryologist. Conversely, no seen senior events (tPB2, cytoplasmic wave, tPN1, tPN2, presence halo, tPNf, disappearance halo) other timings (t2->t10, tM, tSC, tEC, tSB) embryonic cell cycles (ECC1, ECC2, ECC3 s2, s3). Limitations, reasons caution ZymotTM preparation. This device optimises giving rise an optimised population spermatozoa. Future investigations evaluating effect automated samples prepared less technique would potentially yield conversing results. Wider implications findings pilot study demonstrated that gives similar morphokinetics, indicating its effectiveness selection. We expect useful laboratory members helping standardizing Trial registration number NA